How to label Mass Spec Run Information
Information about the Mass Spectrometry used is collected in a google form when data is submitted. Note, an LCMS-metadata file can be used to denote multiple different values for the options below. If such a file is provided, the values entered into the form are used as defaults for any samples that are not included in the file.
A description of each field is listed here:
Mass Spec Run Date
- Date that the sequence was ran on on the machine
Mass Spec Instrument
- The instrument that was used for the run
- Possible values:
- HILIC
- QE
- QE2
- QTOF
Mass Spec Operator
- Researcher who prepared samples for MS and ran them on the machine
LC Method
- Name of the Liquid Chromatography method used
- Possible values:
- unknown
- polar-HILIC-25-min
- polar-reversed-phase-ion-pairing-25-min
- polar-reversed-phase-25-min
- lipid-reversed-phase-25-min
- The submission form only takes predefined values. If the method is not available among the options, select "Other" and enter a description that includes the name and run length. Example:
- lipid-reversed-phase-25-min: This method involves the analysis of lipids using reversed-phase chromatography on C18 columns, coupled with high resolution mass spectrometry. It is mainly used for the analysis of lipids.
Polarity
- Ion mode used for the Mass Spectrometer
- Either "negative" or "positive"