FCirc Rates

Each FCirc calculation is performed per labeled element, and requires the following values/metadata to have been supplied by the researcher:

• Animal Body Weight
• Infusion Rate
• MS Run Date
  • Note: reruns take precedence over previous runs
  • If this date is not provided, and the last serum sample was run multiple times, an arbitrary run is selected
• At least 1 serum sample, with:
  • tissue name containing "serum".
  • collection time
  • A peak group for every tracer the animal was infused with
  • Note: The intact FCirc calculations require the detection of a fully labeled tracer in each of the infused tracers' PeakGroups.

Rates of Appearance/Disappearance (Ra/Rd)

For more information of the variables in the equations below:

• animal_body_weight
• enrichment_fraction
• fraction
• infusion_rate
• tracer_concentration

Intact

Per Gram

• Rd_intact_g = infusion_rate * tracer_concentration / fraction (source)

• Ra_intact_g = Rd_intact_g - infusion_rate * tracer_concentration (source)

Per Animal

• Rd_intact = Rd_intact_g * animal_body_weight (source)

• Ra_intact = Ra_intact_g * animal_body_weight (source)

Average

Per Gram

• Rd_avg_g = infusion_rate * tracer_concentration / enrichment_fraction (source)

• Ra_avg_g = Rd_avg_g - infusion_rate * tracer_concentration (source)

Per Animal

• Rd_avg = Rd_avg_g * animal_body_weight (source)

• Ra_avg = Ra_avg_g * animal_body_weight (source)